Development of novel drug delivery system of bioactive molecules from "cytomedicine" using hybridoma cells entrapped in alginate-poly(L) lysine-alginate microcapsules.
نویسندگان
چکیده
منابع مشابه
Mucoadhesive alginate/poly (L-lysine)/thiolated alginate microcapsules for oral delivery of Lactobacillus salivarius 29.
In this study, thiolated alginate was synthesized by introduction of cysteine to alginate to prepare mucoadhesive alginate/poly (L-lysine)/thiolated alginate (APTA) microcapsules for efficient oral delivery of Lactobacillus salivarius 29 (LS29), a novel therapeutic Lactobacillus strain, in vitro and in vivo. About 759 +/- 32.4 microM of cysteine per gram of alginate was introduced by estimation...
متن کاملSimplified Method of Making Alginate-polylysine Microcapsules for Hybridoma Cell Culture Using Rpm1 1640 Medium
Among various efficient cell culture systems, the encapsulation of living cells within alginate-poly-llysine (PLL) membrane has received much attention as a means of improving the production of desirable cellular products. This method, which was successfully demonstrated by Lim and Moss in 1979, was primarily developed to transplant living cells without an in oivo rejection reaction (Lim and Su...
متن کاملAlginate Polymers for Drug Delivery
Acknowledgements We would like to express our gratitude to everyone who advised and assisted with our research throughout our time at ENSIC. We sincerely appreciate their time and efforts. Abstract The goal of this project was to investigate the properties of alginate stabilized emulsions and their potential role for drug delivery. The polymer acts as a barrier between phases in oil-in-water em...
متن کاملIntraperitoneal distribution of alginate microcapsules in mice
Introduction: Improved drug delivery mechanisms for the treatment of residual peritoneal cancer cells following cytoreduction surgery are needed. Alginate microcapsules are a potentially useful mechanism for delivery of bioengineered cells, but when injected into the peritoneum, their distribution and properties are not well described. Methods: Aliquots of 300, 600 or 1200 microcapsules were in...
متن کاملEnhanced specific antibody productivity of calcium alginate-entrapped hybridoma is cell line-specific.
In order to determine whether the enhanced specific antibody productivity (qMAb) of calcium alginate-entrapped hybridoma is cell line-specific, calcium alginate-entrapped hybridomas (4A2 and DB9G8) were cultivated under the condition where we had previously observed significantly enhanced qMAb of calcium alginate-entrapped S3H5/gamma 2bA2 hybridoma. Unlike S3H5/gamma 2bA2 hybridoma, neither 4A2...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Drug Delivery System
سال: 1997
ISSN: 1881-2732,0913-5006
DOI: 10.2745/dds.12.107